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Orphanin FQ/Nociceptin Activates Nuclear Factor Kappa B In Neuronal Cells
Author(s) -
Donica Courtney L,
Awwad Hibah O,
Wu Huaqing,
Standifer Kelly M
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.962.13
Subject(s) - nociceptin receptor , microbiology and biotechnology , receptor , chemistry , immune system , g protein coupled receptor , nop , sh sy5y , transcription factor , opioid peptide , opioid , biology , cell culture , biochemistry , immunology , neuroblastoma , gene , genetics
Endogenous neuropeptide orphanin FQ/nociceptin (OFQ/N) and its receptor, opioid receptor like‐1 (ORL1; or Nociceptin orphanin peptide receptor, NOP), have been shown to play a modulatory role throughout the body on nociceptive sensitivity, including motor function, spatial learning, smooth muscle tone, cardiac function and the immune system. ORL1 is a G protein coupled receptor (GPCR) that couples to Gi/o proteins and modulates expression and release of inflammatory mediators from immune cells and in the CNS. Inhibitory GPCRs have been shown to activate the immune system regulator, NF κ B. The NF κ B family consists of several subunits, including p105 and p50. When activated, NF κ B translocates to the nucleus and can modify transcription. To determine if OFQ/N modulates NF κ B activity, SH‐SY5Y human neuroblastoma cells were treated with OFQ/N and assessed for changes in nuclear accumulation, DNA binding and protein expression. OFQ/N increases the nuclear accumulation of NF κ B at 30 min and increases the DNA binding of NF κ B by 1 hr as determined by electromobility shift assay (EMSA). Additionally, immunoblot analysis of SH‐SY5Y cell lysates indicates that NF κ B p50 protein expression is up‐regulated by 2 hr. This suggests that OFQ/N may modulate immune system function by activating NF κ B. These studies were supported by DA017380 and OCAST grant HR08‐152 to KMS.