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P2 Receptors and ATP Enhance the Migration of Prostate Cancer Cells
Author(s) -
Peters Wachen,
Sikes Robert A,
Duncan Randall,
Maguire Christine
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.954.7
Subject(s) - purinergic receptor , cell migration , receptor , matrigel , extracellular matrix , microbiology and biotechnology , extracellular , p2 receptor , wound healing , ppads , purinergic signalling , chemistry , lncap , cancer cell , biology , cell , biochemistry , immunology , cancer , adenosine receptor , genetics , agonist
Purinergic signaling stimulates many biological processes. Two classes of purinergic receptors, GPCR (P2Y) and gated channels (P2X), have been identified that bind ATP as a ligand, which can promote cell migration, a critical component of metastasis. ATP also has been shown to have an anticancer effect in vivo. The purpose of this study was to ascertain the effect of ATP on the migration of an isogenic progression series of prostate cancer (PCa) cell lines on three different extracellular matrices. We hypothesize that ATP treatment activates purinergic receptors that increases cell migration on all three extracellular matrices (ECM). RT‐PCR was used to determine the mRNA expression profile of P2 receptors and ecto‐nucleotidases. Migration was examined using wound healing assays as followed by photo microscopy for four days, as well as RT‐PCR to determine effect of interaction with each ECM on the mRNA profile. ATP increased the migration of LNCaP cells on Collagen I to a higher degree than on both Schwann and matrigel over vehicle alone. Peak wound closure occurred after 48 hours. Differential expression of P2 receptors and ectonucleotidases was seen when cells were grown on different matrices. ATP is necessary for PCa migration on collagen I, matrigel and Schwann cell matrix. The differential expression of P2 receptors is speculated to be responsible for variable wound healing on extracellular matrices.