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Elevated Levels of Circulating Microparticles in Disseminated Intravascular Coagulation and Their Impact on the Inflammatory Process
Author(s) -
Rahman Saudur,
Cichon Mark,
Hoppensteadt Debra,
Cunanan Josephine,
Jeske Walter,
Fareed Jawed
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.951.13
Subject(s) - disseminated intravascular coagulation , platelet , coagulation , inflammation , annexin , pathophysiology , annexin a5 , immunology , platelet activation , population , medicine , systemic inflammatory response syndrome , andrology , chemistry , gastroenterology , flow cytometry , sepsis , environmental health
Disseminated intravascular coagulation (DIC) represents one of the most catastrophic coagulopathies, which results in the simultaneous activation of thrombotic and hemorrhagic processes. The pathophysiology of DIC, and the role of inflammation and Microparticles (MPs) is not fully understood. MPs represent small phospholipid‐expressing, procoagulant vesicular fragments, which are released from platelets, leukocytes, and endothelial cells due to cellular disruption and apoptosis. This study was designed to measure functional MPs in 100 random patients from a population of patients diagnosed with DIC. Plasma samples from 30 normal male and female volunteers were used as control. A commercially available functional MP method based on Annexin trapping was used for the determination of the procoagulant activity of MPs (Hyphen Biomedical, Paris, France). The Mean ± Standard Deviation concentration of MPs in the DIC group was 24.6 ± 14.2 nM (Range: 0.0 – 60.0 nM), which was significantly higher than the concentration in the Normal Human Plasma (NHP) control group: 8.5 ± 4.3 nM (Range: 1.3 – 17.4 nM). The distribution curves and the scattergram showed that the MPs concentration in the DIC samples was more widespread than in the NHPs. This study clearly demonstrates that MPs are upregulated in patients with DIC and may mediate the hemostatic activation and inflammatory responses in this syndrome.