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Resveratrol Affects Bone Minerals and Strength in Aging Hindlimb Suspended Rats
Author(s) -
Wright Stephanie,
Jackson Janna R,
Ryan Michael J,
Alway Steve E,
Tou Janet C
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.946.2
Subject(s) - resveratrol , chemistry , femur , medicine , endocrinology , hindlimb , tibia , bone mineral , ashing , zoology , osteoporosis , anatomy , surgery , biology , biochemistry , physics , quantum mechanics
No studies have investigated the role of resveratrol supplementation on age‐related bone loss. Aging is also associated with unloading of bone due to increased bed rest. Hindlimb suspension (HLS) of rats stimulates mechanical unloading of bones. The study objective was to determine the effects of resveratrol supplementation on bones in aging ambulatory (AMB) and HLS rats. Mature adult (6mo) and old (32mo) Fischer Brown Norway male rats were kept AMB or HLS. Rats were than randomly assigned (n=7/group) to received a daily oral gavage of 12.5mg/kg bwt/d trans‐resveratrol or deionized water in 1 milliliter of 0.1% carboxymethylcellulose. At the end of 14 d, the femur and tibia were collected. Bone mineral content (BMC) was measured by ashing at 600°C. Bone calcium (Ca) and phosphorus (P) were determined by inductively coupled plasma optical emission spectrometry. Bone strength measurements were determined by three‐point bending test. In AMB rats, resveratrol supplementation decreased bone strength indicated by reduced tibial ultimate failure energy (p=0.02) in old rats and reduced femoral Young's modulus (YM) (p=0.02) in both mature and old rats. In HLS rats, resveratrol supplementation decreased tibial Ca (p=0.02), P (p=0.02), and strength indicated by lower ultimate stiffness (p=0.04) and YM (p=0.045) in mature rats. Based on our results resveratrol supplementation does not appear to benefit bone.