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The synergistic effect of calcium (Ca), alpha‐linolenic acid (ALA) and conjugated‐linoleic acid (CLA) on osteoblastogenesis and adipogenesis
Author(s) -
Kim Youjin,
Kelly Owen J,
Ilich Jasminka Z
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.939.11
Subject(s) - adipogenesis , conjugated linoleic acid , chemistry , cell growth , calcium , stromal cell , 3t3 l1 , adipose tissue , linoleic acid , biochemistry , endocrinology , medicine , fatty acid , biology , organic chemistry
Recent research points into connections between bone and adipose tissue with major players being Ca, ALA and CLA. The aim of this study was to investigate possible synergistic effects among ALA, CLA and Ca on preosteoblastic MC3T3‐E1, preadipocytic MC3T3‐L1 and stromal ST2 cell proliferation. Low or high (0.5 or 2 mM) Ca in media containing individual or mixture of ALA (8.75–50 μM; n‐6/n‐3=4/1) and CLA isomers (20 μM of c9, t11/t10, c12‐CLAs; 80/10, 90/10, 90/5% respectively) were treated for 6 days (triplicate). Cell proliferation was determined by resazurin salt solution, and florescence was measured at 590 nm. ST2 and 3T3‐E1 cell proliferation was higher at 0.5 mM and lower at 2 mM Ca; Reversed was true for 3T3‐L1 cells (p<0.05). At both Ca levels, individual or mixture of ALA and CLA did not affect 3T3‐L1 cells. In ST2 cells, the effect of ALA was lowered by adding CLA (80/10; 90/10) at 0.5 mM Ca, whereas at 2 mM Ca, ALA+CLA (80/10) further enhanced proliferation. In 3T3‐E1 cells, even though ALA significantly increased proliferation at 0.5mM Ca, its effect was markedly lowered by addition of CLA at all combinations/both Ca levels. Overall, providing high Ca may reduce adipogenesis, but enhance osteoblastogenesis. In a proliferative stage, a synergistic effect among Ca, ALA and CLA on bone/fat cell may not be substantial, but is significant in increasing progenitor stromal cell formation. DMI#1673 and FSU‐CRC#119532 Grant Funding Source : National Dairy Council