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A high fat diet reduces the expression of lipogenic, lipolytic and oxidative genes in white adipose tissue. The effect of the concentration and type of fatty acid is dependent of the dietary protein
Author(s) -
DiazVillaseñor Andrea,
PalaciosGonzalez Berenice,
TovarPalacio Claudia,
Tovar Armando R,
Torres Nimbe
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.938.11
Subject(s) - lipogenesis , adipose tissue , medicine , endocrinology , polyunsaturated fatty acid , white adipose tissue , perilipin , adipocyte protein 2 , biology , chemistry , fatty acid , adiponectin , adipocyte , biochemistry , insulin , insulin resistance
It has been postulated that not only the quantity, but also the type of dietary fat has important implications in the metabolism of lipids and glucose, therefore in obesity. Fatty acids are important mediators of gene expression of metabolic enzymes in the liver, whereas its role in adipose tissue, also involved in metabolism homeostasis, has been little studied. The present study shows that in white adipose tissue of hyperleptinemic and hyperinsulinemic Zucker fa/fa rats chronically fed 5% polyunsaturated fatty acids (PUFA), the expression of genes involved in lipogenesis (SREBP‐1, ACC, FAS, SCD‐1), fatty acid oxidation (PPARα, CPT‐1, UCP‐1, 2 and 3 and AOX) and glucose homeostasis (AMPKα, LKB1 and GLUT4) was dependent by the type of protein consumed (casein or soy), maintaining in both the functionality of the adipocyte. However, if the amount of fat increased in the diet (10%), the expression of all the measured genes was abated drastically, including the lipolytic ones (HSL and perilipin). Gene expression was dependent of the type of fat consumed (PUFA or saturated fatty acids) as well as of the type of protein. Of the four groups tested with 10% of dietary fat, the least affected was the group fed PUFA with soy protein. The action of the different sort of fatty acids in conjunction with the type of protein as regulators of gene expression in the adipose tissue was independent of insulin and leptin. Supported by the INCMNSZ