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Dietary LCPUFA upregulate FADS3 alternative transcripts in neonatal baboon liver
Author(s) -
Reardon Holly T,
Park Woo Jung,
Kothapalli Kumar S.,
Brenna J. Thomas
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.937.4
Subject(s) - fads2 , downregulation and upregulation , docosahexaenoic acid , polyunsaturated fatty acid , baboon , arachidonic acid , endocrinology , eicosapentaenoic acid , biology , medicine , biochemistry , fatty acid , gene , enzyme
BACKGROUND FADS3 shares sequence homology with the fatty acid desaturases FADS1 and FADS2 , but the function of FADS3 is unknown. We have previously described seven FADS3 alternative transcripts (AT) present in both baboons and human cells. This study examined the effect of dietary long chain polyunsaturated fatty acid (LCPUFA) levels on liver FADS3 AT expression. METHODS Twelve baboon neonates were fed low, moderate, or high LCPUFA infant formula from 2–12 weeks of age, when tissues were collected for RNA extraction. Neonates were randomized to three groups: C: Control, no LCPUFA; L: 0.33% docosahexaenoic acid (DHA)/0.67% arachidonic acid (ARA) (w/w); and L3:1.00% DHA/0.67% ARA (w/w). FADS2 and FADS3 AT expression was evaluated by RT‐PCR with normalization to actin. RESULTS As expected, FADS2 was downregulated in the L3 group compared to controls. However, most of the FADS3 AT showed a general trend of upregulation (L3>L>C). CONCLUSION It is well established that dietary LCPUFA downregulate other desaturase genes ( FADS1 , FADS2 , and SCD1 ) in liver. The opposite effect, upregulation of FADS3 AT by dietary LCPUFA, was observed here. This opposing pattern of regulation suggests a dissimilar function for FADS3 AT compared to other FADS gene products. This work was supported by NIH Ruth L. Kirschstein‐NRSA Training Award # 5 T32 HD052471‐03.