Premium
Branched Chain Fatty Acids are taken up and metabolized by Cultured Human Intestinal Cells (Caco‐2)
Author(s) -
RanRessler Rinat Rivka,
Kothapalli Kumar Sesha Durga,
Glahn Raymond P,
Brenna James Thomas
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.937.3
Subject(s) - enterocyte , caco 2 , fatty acid , biochemistry , metabolism , lipid metabolism , gene expression , cell culture , chemistry , microbiology and biotechnology , biology , cell , gene , small intestine , genetics
Background We recently showed differences in the distribution of branched chain fatty acids (BCFA) in the vernix and meconium of healthy newborns: BCFA with < 16 carbons were detected in vernix but not in meconium. In this study we tested if: 1. BCFA are taken up and metabolized by the enterocyte, and 2. BCFA affect gene expression involved in fatty acid (FA) metabolism, using a Caco‐2 cell culture model. Method Uptake study: Caco‐2 cells were grown on 6 well plates for 14 days. Cells were incubated with graded levels of 4 BCFA or control for 4 hours. Cells were harvested for lipid classes and FA analysis. Gene expression study Caco‐2 cells were grown in flasks and incubated for 24 hours with a mixture of 5 BCFA or control at 7 and 14 days post seeding. RNA was reverse‐transcribed into cDNA which was subjected to semi‐quantitative PCR. Results BCFA were taken up by Caco‐2 cells and differentially incorporated into cellular lipid fractions. Small amounts of elongation products were detected. The expression of ELOVL3 (very long chain fatty acid elongase) and BCAT (Branched‐chain amino transferase) was higher at BCFA‐ treated cells at 7 days compared to 14 days post seeding. Conclusion Caco‐2 cells take up BCFA. Their differential incorporation into lipid classes indicates selective metabolism. Expression of BCFA‐related genes is under developmental control when cells are BCFA‐treated. Support: NIH grant T32HD007331