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A Study on the glucose‐regulating Enzyme Activities of Water Extracts from Korea Medicinal Herbs of HepG2 in Hyperglycemic Conditions
Author(s) -
Kim Dae Jung,
Chung Mi Ja,
Seo Dong Joo,
You Jin Kyoun,
Baek Jong Mi,
Kim Jeong Mi,
Choe Myeon
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.932.11
Subject(s) - glucokinase , enzyme , messenger rna , medicine , chemistry , insulin , endocrinology , cell , biochemistry , biology , gene
This study investigated the anti‐diabetic effects on mixture [ Lycii Cortex (JGP), Acanthopanax senticosus (GSO) and Cordyceps militaris (DCH)] of medicinal plant water extracts on glucose‐regulating key enzymes such as glucokinase (GK), pyruvate dehydrogenase(PDH) and acetyl‐CoA carboxylase (ACC). The enzymes were extracted from liver tissue of a type □ diabetic rat (Goto‐kakizaki) rat. The mRNA and protein levels of glucose‐regulating key enzymes measured using RT‐PCR, western blotting and spectrophotometric methods in HepG2 cells or in a cell‐free system. High (4.5g glucose/1L) and low (1g glucose/1L) glucose level media were used for normal and diabetic cell models. GK(306.5%) and ACC(189.6%) activities in the hepatic cytosol fraction by mixture treatment were increased higher than control(100%) but PDH activity was not changed. The HepG2 cells were cultured in the high glucose level media and GK mRNA and protein levels in the mixture‐treated cells for 24 hr were up‐regulated compared to the unexposed control but GK mRNA and protein levels of HepG2 cells in the low glucose level media were slightly lower than the control. Treatment of the mixture significantly reduced ACC mRNA and protein of HepG2 cells in the high and low glucose level media. These observations suggest that the mixture is bio‐material for prevention of type 2 diabetes. (This research was supported by the Ministry of Knowledge Economy through the fostering project of the Human Resource Training Project for Regional Innovation.)