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EFFECT OF OPUNTIA FICUS‐INDICA EXTRACT ON ANTI‐INFLAMMATORY IN MURINE MACROPHAGES
Author(s) -
Jung Jihye,
Shin Jung Hee
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.929.5
Subject(s) - nitric oxide , nitric oxide synthase , traditional medicine , chemistry , macrophage , ficus , anti inflammatory , pharmacology , biochemistry , biology , botany , medicine , in vitro , organic chemistry
Opuntia ficus‐indica is currently consumed for their nutritional properties. Since long time, fruits and stems of many Opuntia species have been used in medicine for burns, wounds, edema, bronchial asthma, hypertension, indigestion, and type II diabetes. Extracts of Opuntia species have been reported to exhibit hypoglycemic, antiulcer, antioxidant, hepatoprotective, and neuroprotective activities. However, less is known regarding inflammatory response properties. In this study, we investgated immunomodulatory activities such as nitric oxide, inducible nitric oxide synthase, cytokines and nuclear factor‐kB in mouse macrophage cells. The extract was manufactured using hot‐water (105°C) and concentrated to 20% as sample. Murine macrophage cells were cultured in RPMI‐1640 supplemented with extract of Opuntia ficus‐indica. With these cells, the amount of nitric oxide, inducible nitric oxide synthase, cytokines and nuclear factor‐kB induced by LPS was analyzed by immunosorbent assay and Western blotting method, respectively. We found that the production of the nitric oxide, inducible nitric oxide synthase, cytokines and nuclear factor‐kB was suppressed when adding the extract during the culture. These results opened the possibility that Opuntia ficus‐indica extract would be applicable to functional food ingredients.