Premium
Impact of selenate treatment on broccoli glucosinolate profile and bioactivity
Author(s) -
Volker Sonja E,
Freeman John,
Banuelos Gary,
Jeffery Elizabeth H
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.928.18
Subject(s) - glucosinolate , chemistry , sulforaphane , selenate , selenium , enzyme , biochemistry , antioxidant , acetylation , enzyme assay , metabolite , botany , biology , brassica , organic chemistry , gene
Selenium (Se)‐enriched broccoli has potent anticarcinogenic effects, which are suggested to be the result of changes in detoxification enzyme activity, induction of apoptosis, and inhibition of histone deacetylation (HDAC) and angiogenesis. We previously showed that feeding high‐Se broccoli powder (H‐SeB; 4.28 ppm) to male Copenhagen rats induced hepatic ethoxyresorufin‐O‐deethylase (EROD) and NAD(P)H:quinone oxidoreductase (NQO1) activity 1.6 and 1.9‐fold over low‐Se broccoli (L‐SeB; 0.25 ppm), respectively. To elucidate the relative role of Se in induction of these enzymes, mouse hepatoma Hepa‐1c1c7 cells were exposed to aqueous extracts of H‐SeB or L‐SeB. Extracts of H‐SeB increased EROD and NQO1 activity 2.7 and 1.3‐fold, respectively. Adding Se as selenite or Se‐methylselenocysteine (SeMSC) to L‐SeB extracts had no impact on EROD or QR activity. Likewise, Lee et al . reported that SeMSC up to 2.5 mM had no direct effect on HDAC in prostate cancer cells. Selenium‐enrichment of broccoli increased levels of the glucosinolate neoglucobrassicin, the hydrolysis product of which is known to induce EROD activity. We conclude that the increase in detoxification enzyme activity is not a direct effect of Se, but the result of a (plant) metabolite of Se and/or changes in the glucosinolate profile. Supported by grants from USDA/AFRI and the Illinois Council on Food and Agricultural Research.