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Broccoli inhibits colon inflammation and carcinogenesis in azoxymethane and dextran sulfate sodium treated mice
Author(s) -
Butler Shan M,
Nho ChuWon,
Pan CheolHo,
Jeffery Elizabeth H
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.928.15
Subject(s) - azoxymethane , inflammation , colorectal cancer , cruciferous vegetables , medicine , endocrinology , carcinogenesis , chemistry , pharmacology , cancer
Inflammation is associated with an increased risk for colorectal cancer through mechanisms that are not well understood. Isothiocyanates, which are the bioactive components in broccoli and other cruciferous vegetables, have been shown to suppress COX‐2 and IL‐6 production from inflammation. The current study was conducted to determine if dietary broccoli can inhibit inflammation‐enhanced colon cancer. Male C57bl/6 mice were fed a 10% broccoli powder diet or standard AIN‐93G diet ad libitum . After a two week feeding period mice were administered a single dose of azoxymethane (AOM; 10mg i.p./kg BW), one week later given 1% dextran sulfate sodium in the drinking water for 7 days. Mice were sacrificed 15 weeks after AOM injection and assessed for tumor incidence and multiplicity, and expression of Proliferating Cell Nuclear Antigen. Dissected colon was fixed using formalin, then paraffin embedded, sectioned and H&E stained. Colon length was significantly greater in broccoli treated mice compared to AIN‐93G treated mice, indicating that broccoli protected against shortening of the colon from inflammation. Dietary broccoli significantly inhibited the formation of adenocarcinomas in the colon compared to AIN‐93G treated mice (p < 0.05). This study suggests that a broccoli diet can slow the progression of inflammation enhanced colon cancer. (Funded in part by the Korea Institute of Science and Technology).