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Inhibitory effects of resveratrol and pterostilbene on human colon cancer cells: a side‐by side comparison
Author(s) -
Nutakul Wasamon,
Sober Hana Shatara,
Qiu Peiju,
Dong Ping,
Xiao Hang
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.928.14
Subject(s) - pterostilbene , apoptosis , resveratrol , cytosol , cancer cell , chemistry , colorectal cancer , cell cycle , cancer , intracellular , cell cycle checkpoint , cancer research , cell , pharmacology , medicine , biochemistry , enzyme
Resveratrol (REV) and pterostilbene (PTS) are structurally related stilbene compounds found in fruits such as grapes and blueberries. We compared their effects on three human colon cancer cells (HT29, HCT116 and Caco2). PTS showed much stronger growth inhibitory effects than REV. IC 50 of PTS were 2~5‐fold lower than those of REV. In HCT116 and HT29 cells, PTS caused cell cycle arrest at G0/G1 and G2/M phases, respectively, while REV did not cause apparent change in cell cycle at same concentrations. In HT29 cells, PTS at 25 μM significantly enhanced apoptosis, while 75 μM of REV was needed to induce same magnitude of apoptosis. In contrast, PTS and REV showed similar potency in inducing apoptosis in HCT116 cells. These pro‐apoptotic effects were further confirmed by increased levels of cleaved PARP protein in cancer cells. Bioavailability is an important factor dictating the biological activity of dietary components. Using HPLC, we determined the cytosol levels of PTS and REV in cancer cells after treatments with two compounds at same concentration. In all three colon cancer cells tested, the cytosol levels of PTS were about 2~3‐fold higher than those of REV. These results indicated that two methyl groups in PTS facilitate the cellular uptake of PTS in comparison with REV. The increased intracellular levels of PTS may contribute considerably to the stronger growth inhibitory effects on colon cancer cells by PTS than by REV. Grant Funding Source : University of Masschuaetts Amherst