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Alpha‐mangostin reduces HT‐29 colon cancer cell proliferation in vitro and inhibits transplantable tumorigenesis in vivo.
Author(s) -
Chitchumroonchokchai Chureeporn,
Suksumrarn Sunit,
Yu Junge,
Clinton Steven K.,
Failla Mark L.
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.928.10
Subject(s) - xanthone , garcinia mangostana , in vivo , in vitro , garcinia , cell growth , chemistry , pharmacology , biology , traditional medicine , biochemistry , medicine , microbiology and biotechnology , stereochemistry
Mangsteen ( Garcinia mangosteen ) is tropical fruit particularly rich in xanthones. The health promoting benefits of mangosteen products have been aggressively marketed despite limited investigation of the bioactivities of the xanthones and particularly in vivo . This study investigated the in vitro and in vivo activity of pure α‐mangostin (αMG), the most abundant xanthone in mangosteen, and a xanthone‐rich crude extract from mangosteen pericarp. Proliferation of HT29 colonic adenocarcinoma cells and growth of HT29 xenografts in nude mice were determined. αMG was a more potent inhibitor of in vitro proliferation of HT29 cells than crude extract (IC 50 = 25 μM vs. 67 μM αMG equivalents [53 μg extract/mL], respectively). Female mice (n=39) were randomly assigned to one of the following diets: AIN‐93G control diet; AIN‐93G diet + 0.1% αMG; and AIN‐93G + 0.2% xanthone‐rich crude extract (~50% αMG). Food intake and growth were not significantly different for the dietary groups after two weeks. Mice then were injected sc with HT29 cells and fed the same diets ad libitum for an additional 3 weeks. Tumor mass was significantly lower in mice fed αMG compared to control diet (524±85 vs 891±106 mg, respectively; P <0.01), but only slightly decreased in rats fed diet with xanthone‐rich extract (808±87: P >0.05). These data support further investigation of αMG and food products containing αMG for the prevention of colon cancer or as complementary therapy. Support : Seed Grant from The OSU CCC and The OSU Center for Advanced Functional Food Research and Entrepreneurship.