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Pharmacological Dose of alpha‐Tocopherol Induces Cardiotoxicity in Wistar Rats Determined by Echocardiography and Histology
Author(s) -
Ferreira Ana Lucia Anjos,
Nascimento Maria Carolina Munhoz Oliveira,
Mastubara Beatriz Bojikian,
Matsubara Luiz Shiguero,
Correa Camila Renata,
Pereira Elenize Jamas,
Moreira Priscila Lucelia,
Carvalho Flavio Augusto,
Burini Caio Henrique Paganini,
Padovani Carlos Roberto,
Yeum KyungJin
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.927.11
Subject(s) - ventricle , ejection fraction , cardiotoxicity , medicine , diastole , tocopherol , cardiac function curve , endocrinology , alpha (finance) , vitamin e , alpha tocopherol , chemistry , heart failure , toxicity , antioxidant , blood pressure , surgery , biochemistry , construct validity , patient satisfaction
The effect of pharmacological dose of alpha‐tocopherol on heart health was determined in Wistar rats. Animals were randomly assigned to either C (control, n = 11) or E (alpha‐tocopherol, n = 11) group. Animals received corn oil (C) or alpha‐tocopherol dissolved in corn oil [250 mg alpha‐tocopherol/(Kg body wt/day)] (E) by gavage for a 7‐week period. Rats underwent echocardiogram and were analyzed for cardiomyocyte histology and cardiac alpha‐tocopherol absorption at the end of study period. As compared to the C group, alpha‐tocopherol supplemented group showed significantly (p<0.05): lower body weight (E, 412.8 g vs C, 480.3 g) and total cardiac weight (E, 0.94 g vs C, 1.08 g); cardiomyocyte histological impairment; smaller left ventricle (LV) (LV end‐diastolic diameter (E, 7.22 mm vs C, 7.37 mm), lower LV systolic [left ventricle fractional shortening (E, 47.6% vs C, 53.6%) and ejection fraction ratio (E, 85.4 vs C, 89.9)] and diastolic [early peak velocities of diastolic transmitral flow (E, 64.6 cm/sec vs C, 75.1 cm/sec)] function. The alpha‐tocopherol uptake in target tissue was confirmed by determination of alpha‐tocopherol concentration medians in cardiac tissue (E, 109.91 nmol/kg vs C, 52.09 nmol/kg). The current study indicates that pharmacological dose of alpha‐tocopherol supplementation can induce cardiotoxicity in healthy rats [Supported by FAPESP 05/52571‐5; USDA1950‐51000‐065‐08S].