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Dietary n‐3 polyunsaturated fatty acids increased oxidative stress in rats with intracerebral hemorrhagic stroke
Author(s) -
Park Yongsoon
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.927.1
Subject(s) - intracerebral hemorrhage , oxidative stress , thiobarbituric acid , polyunsaturated fatty acid , docosahexaenoic acid , medicine , stroke (engine) , chemistry , endocrinology , lipid peroxidation , fatty acid , biochemistry , mechanical engineering , subarachnoid hemorrhage , engineering
The present study was to investigate the hypothesis that EPA and DHA increased the oxidative stress and hemorrhage volume in rats with intracerebral hemorrhagic (ICH) stroke. Thirty five weeks old male rats were fed an AIN‐93M diet containing 0% (n=27), 0.5% (n=15), or 1% EPA+DHA of total calorie for five weeks. Five out of twelve rats fed 1% EPA+DHA (41%) died due to an excessive bleeding within 12 hours after ICH surgery. Behavior test score and hemorrhage volume were significantly (P < 0.05) greater in the 1% EPA+DHA fed rats than other rats. MRI consistently showed that edema and bleeding were visible in only 1% EPA+DHA fed rats. Levels of superoxide dismutase and glutathione were significantly (P < 0.05) lower in 0.5% and 1% EPA+DHA than 0% EPA+DHA fed rats, and thiobarbituric acid reactive substance content was significantly (P < 0.05) higher in 1% EPA+DHA than 0% and 0.5% EPA+DHA fed rats. Level of 8‐hydroxydeoxyguanosine was significantly (P < 0.05) higher in ICH rats with all diets than sham surgery rats. Brain levels of EPA and DHA were highest in rats fed 1% EPA+DHA than 0% and 0.5% EPA+DHA fed rats. These results suggested that intake of 1% EPA+DHA of total calorie could lead to oxidative damage to brain and thus increased the risk of intracerebral hemorrhagic stroke in this rat model. This work was supported by the Korea Research Foundation Grant funded by the Korean Government (KRF‐2008‐313‐C00279).