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Microarray analysis reveals effects on important biological pathways in the liver of mice fed a dietary soy isoflavone concentrate
Author(s) -
Shay Neil F,
Ricketts MarieLouise,
Medrano Theresa I,
Shiverick Kathleen T
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.924.2
Subject(s) - isoflavones , microarray , lipid metabolism , biology , soy protein , cholesterol , microarray analysis techniques , gene expression , endocrinology , food science , gene , medicine , biochemistry
Consumption of soy isoflavones is proposed to lower serum cholesterol levels, improve symptoms of type‐2 diabetes, and lower the incidence of certain types of cancer. This study provides further insight into the effects of a commercially‐available, FDA‐approved, dietary soy isoflavone concentrate (Novasoy® 400) on gene expression profiles in mouse liver. Nude mice, without tumors, were fed a control diet (Advanced Protocol verified casein diet 5K96, Test Diet, Richmond, IN) for 5 days and were then divided into two groups and fed either 1) control diet or 2) control diet supplemented with 0.5% Novasoy® for a further 7 days (n=5 per group). The livers were collected, RNA isolated and oligonucleotide microarray analysis performed using Agilent technology. The soy diet differentially up‐regulated 531 and down‐regulated 469 genes (P < 0.009). Up‐regulated genes were associated with the cell cycle, cell cycle control, cholesterol metabolism, lipid metabolism, lipid and fatty acid transport and detoxification. A significant down‐regulation in genes associated with the stress response, cell proliferation and differentiation were observed. These results demonstrate that a commercially available dietary soy isoflavone concentrate alters gene expression profiles associated with important metabolic pathways to contribute to the beneficial health effects associated with soy consumption.