Premium
Effect of Probiotics on Dietary Energy‐Harvest by Modulation of Intestinal Carbohydrate‐ and Lipid‐Digesting Enzymes
Author(s) -
Cotten Reese,
Vattem Dhiraj A,
Friedman BJ,
Maitin Vatsala
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.923.9
Subject(s) - sucrase , lactobacillus casei , maltase , lipase , food science , firmicutes , bifidobacterium longum , lactobacillus , probiotic , carbohydrate , disaccharidase , chemistry , microbiology and biotechnology , biology , bifidobacterium , biochemistry , enzyme , bacteria , fermentation , gene , genetics , 16s ribosomal rna
Composition of an individual's gut microflora is a recently recognized factor in diet‐related obesity. An obese phenotype has been associated with a relative abundance of the bacterial phylum Firmicutes, resulting in increased ‘energy‐harvest’ or caloric extraction from the diet. Firmicutes are predominantly Clostridium sp. , but include Lactobacillus sp., some of which are probiotics. The objective of this study was to compare ‘energy‐harvesting’ ability of probiotics Lactobacillus casei (Firmicute) with Bifidobacterium longum (Actinobacterium), in terms of effects on intestinal carbohydrate‐ and lipid‐digesting enzymes. Effect of cell‐free supernatants (CFS) from pure cultures of L. casei and B. longum on intestinal disaccharidases maltase and sucrase was investigated in differentiated Caco‐2 cells. Pancreatic lipase activity was measured by an in vitro turbidimetric assay. Un‐inoculated bacterial growth medium was the control. L. casei CFS resulted in an increase in maltase and sucrase activity by 36.7% and 22.6% respectively . B. longum did not significantly alter maltase, and decreased sucrase activity by 12%. Both CFS decreased lipase activity, with a greater decrease in case of L. casei . Overall, the results indicate a greater capacity for caloric‐extraction by L. casei , partially offset by a decrease in lipase activity. Funding source: Texas State University Research Enhancement Program.