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Anti‐inflammatory effects of docosahexaenoyl lysophosphatidylcholine in RAW 264.7 cells and in mice models
Author(s) -
Kim MinJee,
Sohn MiRan,
Sok DaiEun,
Kim Mee Ree
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.923.3
Subject(s) - lysophosphatidylcholine , zymosan , docosahexaenoic acid , chemistry , in vivo , biochemistry , pharmacology , in vitro , biology , polyunsaturated fatty acid , fatty acid , phospholipid , phosphatidylcholine , microbiology and biotechnology , membrane
Lysophosphatidylcholines(lysoPC) have been known to play a role as lipid mediators in various cellular responses. However, it is not clear whether polyunsaturated acyl group in the structure of lysoPC is required for the cellular function. Nonetheless, lysoPCs with polyunsaturated acyl group are known to be abundant in plant and animal sources. In this regard, we examined the anti‐inflammatory effects of lysoPCs. In this study, we examined the an ti‐inflammatory effects of various lysophosphatidylcholine (lysoPC) containing linoleoyl, arachidonoyl or docosahexaenoyl group in RAW 264.7 cells or in vivo. First, arachidonoyl lysoPC and docosahexaenoyl lysoPC were found to inhibit LPS‐induced nitro oxide production in RAW 264.7 cells, with IC 50 values of 0.38 μM and 1.90 μM, respectively. However, pre‐incub ation with linoleoyl lysoPC up to 50 μM was not found to inh ibit the LPS‐induced nitro oxide production in RAW 264.7 ce lls. Idditionally, I.V. injection of docosahexaenoyl lysoPC suppres sed the Zymosan‐induced plasma leakage in ICR mice with IC 50 value of 50 μg/kg and maximal inhibition around 80%. In contrast, arachidonoyl lysoPC was observed to inhibit Zymosan‐in duced plasma leakage in ICR mice less potently, and linoleoy l lysoPC (150 μg/kg) was failed to inhibit Zymosan‐induced pl asma leakage in ICR mice. In a separate experiment, where do cosahexaenoyl lysoPC peroxide and docosahexaenoic acid were tested for the effect on Zymosan‐induced plasma leakage, 17( S )‐hydroperoxy‐4,7,10,13,15,19‐docosahexaenoyl lysoPC and docos ahexaenoic acid were failed to inhibit t Zymosan‐induced plas ma leakage in ICR mice. From these results, it is proposed that d ocosahexaenoyl lysoPC displays potent anti‐inflammatory effects and this may participate in the regulation of LOX activity in biological systems.