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Inhibitory Effects of Rhaponticum uniflorum Extract Fractions on the Biosynthesis of Inflammatory Mediators
Author(s) -
Kim Gun Hee,
Hwang Keum Hee,
Lee JeHyuk
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.922.13
Subject(s) - ethyl acetate , chemistry , antioxidant , chloroform , dpph , anti inflammatory , nitrite , butanol , biochemistry , prostaglandin , pharmacology , ethanol , chromatography , food science , biology , nitrate , organic chemistry
Rhapontici radix (RR), the root part of Rhaponticum uniflorum (L.) DC , have been widely used as a traditional food material and medicine for the treatment of chronic gastritis, alleviation of pain, and immunological improvements in Korea. Total flavonoids took approximately 14–82% of total phenolic acids in RR ‐ extract fractions. DPPH radical scavenging activity of ethyl acetate, butanol, aquious‐fraction was superior to those of other fractions. Especially ethyl acetate‐fraction of RR had a potent reducing power, producing reductones. All RR‐extract fractions inhibited LPS‐induced nitrite production in a dose‐dependent manner. Hexane‐ and chloroform‐fraction (25 μg/mL) suppressed LPS‐induced nitrite production below basal level in macrophage cells. And RR‐extract fractions did not show NO‐radical scavenging activities chemically all most, hexane‐ and chloroform‐fraction (25 μg/mL) suppressed approximately 68 and 75% iNOS mRNA transcription, respectively. Otherwise, COX‐2 mRNA transcription was not affected by extract‐fractions of RR. Production of prostaglandin E 2 , interleukin‐1β, and ‐6, which are inflammatory mediators, were inhibited significantly by extract‐fractions of RR. Based on these results, R. uniflorum‐ extract may be useful as an alternative medicine for the relief and retardation of immunological inflammatory responses through the reduction of inflammatory mediators.