Premium
Delphinidin Alters Hydrogen Peroxide Induced Cytotoxicity and Apoptosis in Human Colon Adenocarcinoma Cells
Author(s) -
Tokarev Yulia,
Fischer Joan G
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.921.9
Subject(s) - delphinidin , apoptosis , viability assay , hydrogen peroxide , cytotoxicity , chemistry , mtt assay , microbiology and biotechnology , antioxidant , biochemistry , in vitro , biology , cyanidin
Delphinidin has been shown to induce apoptosis in cancer cells at high concentrations while protecting normal cells at low concentrations. The objective of this research was to determine the effect of lower levels (1–25 μmol/L) of delphinidin on cell viability and apoptosis in hydrogen peroxide stressed HT‐29 human adenocarcinoma cells. Cells, incubated at 37ºC, 5% CO2, 95% air, were pre‐treated for 4 h with 1, 5, 10, and 25 μmol/L delphinidin before being stressed with 100 μmol/L H 2 O 2 for 2 h. Cell viability was determined using the MTT assay 36 h after treatment. Delphinidin had a protective effect against H 2 O 2 induced cytotoxicity, significantly (p< 0.05) increasing cell viability at 1–10 μmol/L compared to H 2 O 2 alone, with highest increase found at 1 μmol/L. This suggests an antioxidant effect at low concentrations. Apoptosis was assessed by measuring caspase‐3 activity 48 h after treatment. While it was hypothesized that an increase in cell viability would be associated with a decrease in caspase‐3 activity, our initial results suggested an increase in caspase‐3 activity from 1 – 10 μmol/L delphinidin, although this was only significant at the 10 μmol/L concentration (p < 0.05). Funding: GA‐AES.