Premium
Antioxidant capacities of seven flavonoid compounds isolated from pulp of acai fruit (Euterpe oleracea)
Author(s) -
Kang Jie,
Wu Tong,
Li Zhimin,
Jensen Gitte S,
Schauss Alexander G,
Wu Xianli
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.921.8
Subject(s) - oxygen radical absorbance capacity , vitexin , chemistry , orientin , antioxidant , luteolin , reactive oxygen species , flavonoid , food science , pulp (tooth) , hydrogen peroxide , quercetin , biochemistry , antioxidant capacity , medicine , pathology
The pulp of açai fruit ( Euterpe oleracea Mart.) has been demonstrated to exhibit extremely high antioxidant capacity. Seven major flavonoids were isolated from freeze‐dried acai pulp by various chromatographic methods. Their structures were elucidated as orientin ( 1 ), homoorientin ( 2 ), vitexin ( 3 ), luteolin( 4 ), chrysoeriol ( 5 ), quercetin ( 6 ), and dihydrokaempferol ( 7 ) by NMR, MS and compared to authentic standards. Antioxidant capacities of these flavonoids were evaluated by the following assays: oxygen radical absorbance capacity (ORAC); cell‐based antioxidant protection in erythrocyte (CAP‐e); ROS formation in polymorphonuclear granulocytes (ROS PMN). ORAC values varied widely (1,423.6 – 14,817.4 μmol TE/g) among the seven compounds based on numbers and positions of hydroxyl groups and/or substitute groups. The ORAC values of aglycones were generally higher than that of glycosides. CAP‐e results indicated that three compounds ( 4, 6, 7 ) could enter the cytosol and significantly reduce oxidative damage within living cells. ROS PMN assay showed four compounds ( 2, 3, 7, 6 ) had exceptional anti‐inflammatory properties in reducing ROS formation in PMN cells, even when high amounts of ROS were produced upon hydrogen peroxide administration. Supported by MonaVie LLC.