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Cytoprotective activity of leaf crude extract of Eugenia uniflora L. and quercetin in insulin‐producing cells
Author(s) -
Oliveira Emilio Henriette Rosa,
Braatz Simoní Marinéia,
Felipe Elise Tatiane,
Oliveira Márcia Regina Paes,
Kanunfre Carla Cristine,
Carpinelli Angelo Rafael,
Curi Rui
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.921.12
Subject(s) - cytoprotection , quercetin , viability assay , oxidative stress , chemistry , antioxidant , traditional medicine , biochemistry , pharmacology , cell , medicine
Eugenia uniflora L. is used in popular medicine as an alternative to the treatment of this disease because they are rich in phenolic compounds. In this study was evaluated the cytoprotective activity of leaf crude extracts and quercetin by HE stain and by MTT assay. Acute and chronic assay were performed through pre‐incubation of RINm5f cells for 1h or 24 h, and posterior induction of stress by H 2 O 2 (150μM/2h). Acute essay showed a reduction in the viability of the control group of 33%. Cytoprotection was partially achieved with aqueous extract (50μg/mL) and quercetin (6.25 > 3.13 μg/mL). Chronic essay showed a reduction in the viability of the control group of 45%. The cytoprotection achieved with aqueous and alcoholic extract was about 16.5% and 18%, respectively. The cytoprotection achieved with quercetin was 12% (6.25μg/mL) and 25% (3.13μg/mL). Through HE stain was verified loss of intercellular adhesion and membrane and shape cell alterations. Aqueous extract showed partial prevention in acute essay. There was a more intense prevention of damage by 3.13 μg/mL quercetin. At chronic essay, aqueous extract (75μg/mL) and quercetin (3.13μg/mL and 6.25 μg/mL) partially prevented morfologic damage. These effects suggest that the tested compounds reduce oxidative stress, probably by their direct (acute essay) and indirect (chronic essay) antioxidant activity. Supported by Araucaria Foundation.