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Effects of selenium source on blood selenium content, blood cell counts and peripheral blood mononuclear cell mRNA profiles in maturing beef heifers
Author(s) -
Brennan Kristen M,
Burris Walter R,
Boling James A,
Matthews James C
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.916.4
Subject(s) - selenium , peripheral blood mononuclear cell , endocrinology , medicine , chemistry , red blood cell , whole blood , immune system , blood cell , andrology , white blood cell , biology , immunology , biochemistry , in vitro , organic chemistry
To determine if source of dietary selenium (Se) supplementation affects blood Se content and expression of biomarkers of immune status in blood cells of growing cattle, Angus heifers were assigned (n = 10) to 4 Se treatments (TRT) for 126 d. The basal diet contained 0.08 mg Se/d, whereas the mineral supplements provided no (control, C), or 3 mg Se/d as inorganic (sodium selenite, SS), organic (Sel‐Plex ® , SP), or a 50/50 mix (SS/SP). Jugular blood was collected for blood cell counts, Se analysis and peripheral blood mononuclear cell (PBMC) isolation. Whole blood (WB), plasma, and red blood cell (RBC) Se content was greater (P < 0.01) for all TRT versus C. SS/SP and SP heifers had greater (P < 0.01) WB and RBC Se content than SS, while plasma Se tended (P = 0.06) to be higher in SS/SP heifers than SS. PBMC count was negatively correlated (P < 0.05) to plasma and WB Se content in SP heifers, and tended (P = 0.06) to be negatively correlated to WB Se in SS/SP heifers. Se TRT did not affect interleukins 1β, 8, and receptor 2α mRNA levels in PBMC, while selenoprotein S (SelS) mRNA content was decreased (P < 0.05) in SS/SP and SP versus C heifers. Because SelS plays an important role in the regulation of the inflammatory cytokine response, decreased SelS mRNA levels indicate supplementing with 3 mg/d SP or SS/SP may result in cattle with a less challenged immune state. This work is supported by the Alltech‐University of KY Animal Nutrigenomics Alliance.