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In vivo enrichment of chicken eggs with 14C‐B12 for determining vitamin B12 bioavailability in humans
Author(s) -
Garrod Marjorie G,
Miller Joshua W.,
Calvert Christopher C.,
Buchholz Bruce A.,
Green Ralph,
Johnson Heidi A.,
Allen Lindsay H.
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.915.12
Subject(s) - bioavailability , vitamin b12 , in vivo , feces , chemistry , food science , urine , biology , zoology , pharmacology , biochemistry , microbiology and biotechnology
Animal source foods such as eggs are the only natural dietary source of vitamin B12 but there is limited information on its bioavailability from these sources. The objective was to test the feasibility of enriching eggs in vivo with sufficient 14 C‐B12 for use in human bioavailability studies. 14 C‐B12 was biosynthesized using Salmonella enterica , purified and injected into the thigh muscle of a laying hen. Eggs were collected 21 d, pooled, scrambled and cooked. A serving of egg containing ~0.7 μg 14 C‐B12 (~30 nCi) was fed to each of 10 healthy humans after an overnight fast. 14 C, detected by accelerator mass spectrometry, first appeared in plasma at 3–4 h after dose and peaked at 7–10 h, with 5–40% appearing in urine in the first 24 h. Fecal 14 C analyses in progress will enable calculation of % bioavailability of B12. We conclude that this protocol can be used for human B12 bioavailability studies. Funded by the American Egg Board.