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Investigation of class I integron presence and subsequent variable region characterization of 15 clinical isolates from Buenos Aires, Argentina
Author(s) -
Kim Jun,
Mercado Jeffrey,
Centron Daniela,
Tolmasky Marcelo
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.909.1
Subject(s) - integron , biology , genetics , gene , microbiology and biotechnology , escherichia coli
The aim of this study was to detect class I integron presence and characterize variable regions in 15 clinical isolates from Buenos Aires. Through PCR amplification of 15 isolates of various Enterobacteriaceae, 60% (9/15) were positive for the class I integron structure. NCBI Blast sequencing of 38 sequences revealed that 26% (10/38) contained a segment encoding the aac(6′)‐Ib and 21% (8/38) frequency of occurrence for the aadA1 gene cassette. Several studies have concluded that integron bearing isolates have a significant association with multi‐resistance and this conclusion is supported by our findings.