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Repressive effect of polyunsaturated fatty acids on serine dehydratase expression in rat hepatocytes
Author(s) -
Contreras Alejandra,
Ortiz Victor,
Torres Nimbe,
Tovar Armando R
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.894.2
Subject(s) - linoleic acid , biochemistry , polyunsaturated fatty acid , peroxisome , palmitic acid , fatty acid , chemistry , free fatty acid receptor , oleic acid , biology , receptor
Peroxisome proliferator‐activated receptor alpha (PPARα) is activated by ligands such as polyunsaturated fatty acids (PUFAs) and regulates expression of enzymes involved in fatty acid oxidation. Recently, it was found that PPARα also regulates the metabolism of amino acids in liver, however, the knowledge of molecular mechanisms of amino acid regulation in liver is limited. To assess the effect of natural (linoleic acid, LA) and synthetic PPARα ligands (Wy14643) on the expression of the amino acid degrading enzyme serine dehydratase (SHD) and the enzyme involved in fatty acid oxidation carnitine palmitoyl transferase‐1 (CPT‐1), rat hepatocytes were incubated with the agonist Wy14643, palmitic acid (saturated fatty acid) or linoleic acid (PUFA), and gene expression was measured. Addition of forskolin to the medium increased SDH gene expression as expected but did not altered CPT‐1 expression. However, addition of linoleic acid and palmitic acid to the medium increased CPT‐1 by 100% and 40%, but repressed SDH gene expression by 53% and 23% respectively. These data indicated that the insaturated linoleic acid reduced amino acid catabolism and stimulated fatty acid oxidation through PPARα in a higher extent than the saturated palmitic acid. Supported by CONACYT and INCMNSZ.