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The effect of p66Shc on energy expenditure in mice
Author(s) -
Stern Jennifer H,
Hagopian Kevork,
McDonald Roger B,
Ramsey Jon J
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.888.7
Subject(s) - chemistry , medicine , energy expenditure , endocrinology , metabolism , knockout mouse , energy metabolism , biology , receptor , biochemistry
Previous investigations have shown that the p66 Shc protein has a significant effect on life span. While the mechanism underlying the impact that p66 Shc has on life span remains to be elucidated, it has been proposed that p66 Shc may interact with the IGF‐1/insulin signaling pathway to modulate metabolism, and possibly energy expenditure, under conditions of stress. As a first step in testing this possibility, oxygen consumption and carbon dioxide production were measured in 3‐month‐old male p66 Shc knockout (p66 Shc−/− ) and wild type C57BL/6 mice for 24h at 23 o C and for 5h at 12–13 o C. Average 24h energy expenditure (EE) at 23 o C did not differ (P > 0.10) between the p66 Shc−/− and C57BL/6 groups. Dark vs. light phase EE (12h dark: 12h light) was greater in both groups, but did not differ among p66 Shc−/− and C57BL/6 mice. Cold‐exposed EE also did not differ between the groups. RQ measured at 23 o C was higher in the p66 Shc−/− compared to C57BL/6 mice and this difference was most pronounced during the dark cycle. Cold‐exposed RQ was also greater in the p66 Shc−/− vs. C57BL/6 mice (p66 Shc−/− , 0.85 ± 0.004; C57BL/6, 0.83 ± 0.005). These preliminary data suggest that while EE does not differ in p66 Shc−/− vs. C57BL/6 mice, the mix of substrate used for energy metabolism may be influenced by the p66 Shc protein. The influence of the p66 Shc protein on substrate utilization observed here is consistent with a role for this protein in the IGF‐1/insulin‐dependent intracellular signally pathway. (Supported by NIH PO1 AG025532)