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The Role of Wnt‐10b in Osteoblast Development
Author(s) -
Chase Jennifer A,
Moedder Ulrike,
Monroe David,
Khosla Sundeep
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.888.1
Subject(s) - wnt signaling pathway , osteoblast , microbiology and biotechnology , stromal cell , bone morphogenetic protein 2 , lrp5 , biology , chemistry , cancer research , signal transduction , genetics , in vitro
Wnt signaling plays a central role in the development of bone forming osteoblasts and bone remodeling. This study analyzed Wnt‐10b control of gene expression throughout osteoblast differentiation. Genes regulated by Wnt‐10b were identified by analyzing five mouse osteoblast lines (C3H10T1/2, ST‐2, bone marrow stromal cells, MC‐3T3, and calvarial osteoblasts). These cell lines were exposed to media from a Wnt‐10b producing cell line via a transwell system. mRNA levels of 32 osteoblastic, Wnt and BMP responsive genes were quantified by qPCR. Several genes were consistently up/down regulated, representing different stages of osteoblast commitment and differentiation. In the second part of the study, C3H10T1/2 cells were treated with BMP2 osteoblast differentiation media to stimulate the growth of osteoblastic cells. The control and Wnt‐10b treated cells were harvested at 0, 6, 9, 12, or 15 days to observe the effect of Wnt‐10b during differentiation. Analysis of 64 genes showed two known Wnt‐3a targets, complement component 3 (C3) and chemokine ligand 16 (Cxcl16), were highly regulated by Wnt‐10b. This study indentifies a number of important genes that are regulated by Wnt‐10b during osteoblast differentiation. This project was funded by the Mayo Graduate School and the Summer Undergraduate Research Fellowship.