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Toward the isolation of a novel RNA‐RNA interaction based on an activity of a trans ‐acting ribozyme
Author(s) -
Ishikawa Junya,
Furuta Hiroyuki,
Ikawa Yoshiya
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.882.2
Subject(s) - ribozyme , rna , ligase ribozyme , digital subscriber line , riboswitch , computational biology , crispr , chemistry , modular design , rna ligase , stereochemistry , biology , combinatorial chemistry , genetics , biochemistry , non coding rna , computer science , gene , telecommunications , operating system
The DSL ribozyme, a class of artificial ligase ribozymes, has a highly modular architecture, which catalyzes template‐directed RNA ligation on a helical substrate that can be either covalently connected ( cis ‐DSL) or physically separated ( trans ‐DSL) from the catalytic module. Substrate recognition by the catalytic module is promoted by one or two sets of GNRA/receptor interactions acting as clamps in the cis or trans configurations, respectively. In the field of organic chemistry, modular functional groups are attached into parent compounds to generate new or improved properties. This strategy is well established and a variety of the mudular groups renders the number of compounds infinite. Along with the fact, an abundance of RNA motifs could expand possibilities of functional RNAs. Recent works demonstrated artificially designed RNAs composing naturally occurring structural motifs could be a scaffold to isolate a small RNA unit. In the present study, we are attempting to isolate an artificial loop receptor motif based on the activity of trans ‐DSL. For the selection, we have constructed a new derivative of the trans ‐DSL by replacing one of its clamp modules with a target loop and a random sequence. This research was supported by Grant‐in‐Aids on Innovative Areas “Emergence in Chemistry” [No. 21111518 to Y.I.] and for the Global COE program, “Science for Future Molecular Systems” [to H.F.] from The MEXT of Japan.