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Diacylglycerol production at the Golgi: regulation by Ca 2+
Author(s) -
Kunkel Maya,
Newton Alexandra
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.873.1
Subject(s) - diacylglycerol kinase , golgi apparatus , microbiology and biotechnology , protein kinase c , intracellular , endoplasmic reticulum , biology , signal transduction , protein kinase a , chemistry , kinase
Protein kinase C and protein kinase D are potently activated by agonist‐evoked increases in diacylglycerol. Using live cell‐imaging probes for kinase activity, we have previously shown that both kinases are robustly active at the Golgi following stimulation of Gq‐coupled receptor signaling pathways, displaying activation signatures at the Golgi that are distinct from those at the plasma membrane. Here we address the mechanism by which signals received at the plasma membrane cause these two protein kinases to be activated at the Golgi. Using FRET‐based reporters to image diacylglycerol production, we show that Ca 2+ is necessary and sufficient to transduce signals from the plasma membrane to the Golgi. First, chelation of intracellular Ca 2+ prevents UTP‐dependent increases in diacylglycerol at the Golgi, but not plasma membrane. Second, raising intracellular Ca 2+ by treating cells with thapsigargin induces diacylglycerol production at the Golgi. Thus, agonist‐evoked increases in intracellular Ca 2+ cause increases in Golgi diacylglycerol, allowing this intracellular membrane to serve as a platform for signaling by protein kinases C and D. This work was supported by NIH GM43154 and NIH DK54441.