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Effect of TBT exposures on Protein kinase D activity of human Natural Killer cells
Author(s) -
Rana Krupa,
Whalen Margaret M.
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.855.6
Subject(s) - tributyltin , kinase , protein kinase a , mapk/erk pathway , immune system , chemistry , antibody , microbiology and biotechnology , biology , immunology , organic chemistry
Natural killer (NK) cells are lymphocytes that target and destroy (lyse) tumor cells, virally infected cells, and antibody coated cells. Due to their capacity to respond without a need for prior sensitization, they are at the forefront of the immune response to tumor and virally infected cells. Tributyltin (TBT) is an organotin that is found in many different products. It is found in antifouling paints and has been used as an antifungal agent in a wide variety of applications including silicon baking parchments and shower curtains. Due to its contamination of the environment it has also been found in human blood. Exposure of human NK cells to TBT decreases their lytic function and causes activation of mitogen activated protein kinase (MAPK). The aim of the current study is to examine the role of the serine threonine kinase, protein kinase D (PKD) in the TBT‐induced activation of NK cell MAPKs. PKD activation has been shown in other cells types to lead to the activation of MAPKs. In this study NK cells were exposed to 300, 200, 100, 50, and 25 nM TBT for 10 min and 1 h and cell lysates were prepared and analyzed by SDS‐PAGE followed by Western blot. The activation state of PKD was monitored using an antibody to phospho‐PKD. The results indicated that PKD was activated approximately two fold by 300‐100 nM TBT within 10 min. These results suggest that TBT‐induced activation of PKD may be in part responsible for the TBT‐induced activation of MAPK. Supported by NIH grant S06 GM008092‐34.