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A novel vision to the process of ERpahgy in yeast
Author(s) -
Liu AngYu,
Ho KungHsien,
Huang WeiPang
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.853.4
Subject(s) - autophagy , vacuole , endoplasmic reticulum , microbiology and biotechnology , cytoplasm , unfolded protein response , cytosol , proteostasis , organelle , dithiothreitol , chemistry , biology , biochemistry , apoptosis , enzyme
Under oxidative stresses, accumulation of misfolded protein in the endoplasmic reticulum (ER) activates downstream pathway ‐ the unfolded protein response (UPR) to protect cells. Autopahgy, on the other hand, is another protective response to cope with cellular stresses in eukaryotes, which involves in the degradation of cytosolic proteins and defective organelles. It is known that the ER‐selective autophagy, or ERphagy, will be induced during ER stresses and serve a protective role to maintain cell survival. However, the correlation of ERphagy with other type of autophagy is not clear. Here, we show that ERphagy is a selective process distinct from the cytoplasm‐to‐vacuole targeting (Cvt) pathway and the non‐selective macroautophagy. We found that under the dithiothreitol (DTT)‐induced ER stress, the ER‐containing autophagosomes (ERAs) would tend to accumulate in cytoplasm rather than fuse with vacuoles rapidly. However, soon after DTT is removed, ERAs will start to fuse with vacuoles and be degraded. We also discovered that the Cvt pathway would be partially inhibited at the vesicle forming stage during ERphagy, causing the delayed maturation phenotype of prApe1. Nevertheless, ERphagy seems to have limited effect on the process of the starvation–induced macroautophagy, and starvation treatment would not promote the clearance of accumulated ERAs in cytoplasm either. Taken these together, ERphagy as a selective process may compete with the Cvt pathway for utilizing the share of common regulatory machinery, but not preferable to the starvation‐induced macroautophagy. NSC96‐2311‐B‐002‐012‐MY3