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A comparative study of the lipid composition of the brain of chicken and rat during myelination; A chromatographic‐densitometric analysis.
Author(s) -
Helmy Fatma Mahmoud,
Morris Aaron
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.851.2
Subject(s) - sphingomyelin , glycolipid , densitometry , phospholipid , embryo , endocrinology , medicine , fetus , biology , cerebroside , phosphatidyl choline , thin layer chromatography , central nervous system , andrology , chemistry , biochemistry , chromatography , cholesterol , microbiology and biotechnology , pregnancy , membrane , genetics
The lipid profile of brain from 10 day and 18 day old chick embryo, one day old chick and adult chicken (10 weeks old), as well as full‐term fetus rat, 21 day old male rat, young adult male rat, and pregnant female rat brain; were analyzed by thin layer chromatography and densitometry. 10‐day old chick embryo brain revealed a low concentration of GDG, n‐CMH, h‐CMH, and CMH‐S. The concentration of these glycolipids increased gradually as the chick embryo advanced in development, indicating age relatedness, and reached the highest level in the brain of adult chicken. Rat brain did not begin to show the presence of these lipids until at least 21 days old, possibly indicating that the nervous system of the bird model develops faster than that of mammals. In addition, phosphatidyl ethanolamine plasmalogen (PE), was shown to be the only alkenyl phospholipid in all samples analyzed. Two molecular species have been identified (PE 1 and PE 2 ), and PE 2 has been shown to correlate with the myelination process. Phosphatidyl choline (PC), and sphingomyelin (SM), were also present. SM concentration increased gradually during development, and reached its highest level in adult chicken brain, as in rat brain. The correlation of myelination, brain development and the level of these glycolipids, indicated the important role of these glycolipids in both brain maturation and function.