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17β‐Estradiol inhibits isoproterenol activated lipolysis in murine adipocytes
Author(s) -
Tagawa Noriko,
Mitsumasu Satoshi,
Yuda Ryosuke,
Kobayashi Yoshiharu
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.849.7
Subject(s) - lipolysis , endocrinology , medicine , chemistry , phosphodiesterase , hormone sensitive lipase , yohimbine , adipose tissue , receptor , biology , biochemistry , enzyme , antagonist
Stress stimulates sympatho‐adrenomedullary activity and lipolysis in adipocytes. Increase in plasma free fatty acids has been demonstrated to be a major link between obesity and insulin resistance. However, there are many reports describing the anti‐obesity effect of estrogen. This study investigated the effect of estrogen on isoproterenol (ISO) activated lipolysis in murine adipocytes. ISO (5 μM) and 17β‐Estradiol (E 2 ) was added to mature 3T3‐L1 adipocytes in 6 well plates. After 2 hr incubation at 37 °C in 5% CO 2 /95% air, the glycerol concentration in DMEM medium was measured. Effects of β 2 ‐adrenoceptor inhibitor (yohimbine, 50 μM), phosphodiesterase inhibitor (cilostamide, 2 μM) and estrogen receptor blocker (tamoxifen, 10 μM) on the inhibitory effect of lipolysis of E 2 were also tested. Further, effect of E 2 on cAMP production and hormone sensitive lipase (HSL) activity in 3T3‐L1 adipocytes were investigated. E 2 (5–50 μM) inhibited ISO activated lipolysis in 3T3‐L1 adipocytes dose‐dependently. Yohimbine, cilostamide and tamoxifen did not abolish the inhibitory effect of E 2 on ISO activated lipolysis. E 2 inhibited HSL activity, whereas E 2 did not reverse the increased cAMP level induced by ISO stimulation. These results suggest that E 2 exerts its inhibitory effect of ISO activated lipolysis by inhibition somewhere down‐stream of cAMP production but up‐stream of the HSL signaling pathway.