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Extraction and evaluation of ganglioside‐like material in Lactobacillus bacterium
Author(s) -
Sawdon Alicia J,
Ettinger Anna C,
Moore Kathleen H
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.847.1
Subject(s) - ganglioside , sialic acid , chemistry , chromatography , lactic acid , centrifugation , biochemistry , membrane , bacteria , biology , genetics
Previous work has shown that many cultured dairy products are rich in gangliosides that may confer antimicrobial effects. It is unclear whether lactic acid bacteria (LAB) used to culture dairy products contain gangliosides; this effort seeks to isolate and characterize gangliosides from LAB membranes. LAB were grown in a ganglioside‐free thioglycolate medium. Cells were harvested by centrifugation and washed extensively with saline. After suspension in a 1:1 chloroform/methanol solution, cells were sonicated to disrupt cell walls and membranes. Subsequent Folch‐like fractions were separated from other lipid components by anion exchange chromatography into potential ganglioside pools. Sialic acid‐specific resorcinol reagent was used to screen chromatographic fractions for possible ganglioside content; additionally, the column fractions were analyzed by thin layer chromatography against ganglioside and sialic acid standards. Initial experiments with an LAB species have yielded a compound with ganglioside‐like mobility (R F = 0.34) and color response. Characterization studies include reactivity with N‐acetyl neuraminidase, as well as effect of exogenous sialic acid on production of ganglioside‐like material. The probiotic effect of LAB may relate to the presence of gangliosides. Supported by the Provost's Undergraduate Student Research Award.