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Regulation and physiological roles of the phospholipase B's encoded by PLB1‐3 in Saccharomyces cerevisiae
Author(s) -
Surlow Beth,
Bishop Andrew,
PattonVogt Jana
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.846.1
Subject(s) - extracellular , saccharomyces cerevisiae , mutant , microbiology and biotechnology , intracellular , phospholipase , actin cytoskeleton , biology , biochemistry , phospholipid , phospholipase d , downregulation and upregulation , periplasmic space , signal transduction , chemistry , cell , gene , cytoskeleton , enzyme , escherichia coli , membrane
The phospholipase Bs (PLBs) encoded by PLB1, PLB2 , and PLB3 , are associated with the plasma membrane, cell wall, and periplasmic space of Saccharomyces cerevisiae . PLB activity catalyzes phospholipid deacylation and the concomitant production of intracellular and extracellular glycerophosphodiesters.. The extracellular glycerophosphodiesters produced, glycerophosphocholine and glycerophosphoinositol, are transported into the cell via the permease encoded by the GIT1 gene. Whereas GIT1 is transcriptionally regulated by phosphate levels, we report here that PLB1‐3 are not similarly regulated. Alterations in RAS/cAMP signaling, however, do affect expression of PLB1‐3; PLB1 and PLB3 message levels are increased upon upregulation of RAS/cAMP signaling, whereas PLB2 message levels are decreased. The mechanism of this regulation is under study. To probe the physiological roles of Plb1‐3‐mediated phospholipid turnover, we performed phenotypic analyses. A strain in which PLB1‐3 have been deleted (a plb1‐3Δ mutant) exhibits slower growth as compared to its wild type counterpart. In addition, a plb1‐3Δ mutant exhibits increased latrunculin A sensitivity as compared to wild type, suggesting a connection between cell‐surface phospholipase B activity and stability of the actin cytoskeleton. Current studies are aimed at determining the molecular mechanisms involved in producing the observed phenotypes.