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Saccharomyces cerevisiae responds to cold shock by inducing the transcription of ribosome biogenesis genes
Author(s) -
Infanzon Bianca A,
Buckmelter Kristen M,
Liu Elizabeth M,
Sakhon Olivia S,
Citti Wesley T,
Dahlquist Kam D
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.833.13
Subject(s) - saccharomyces cerevisiae , ribosome biogenesis , gene , cold shock domain , biology , gene expression , microbiology and biotechnology , genetics , ribosome , rna
Previous studies on the global transcriptional response of budding yeast, Saccharomyces cerevisiae , to cold shock have revealed that the response can be divided into a set of early response genes (after 15 minutes to 2 hours of cold temperatures) and late response genes (after 12 to 60 hours of cold temperatures). The late response genes include the ESR genes induced by many environmental stresses, but less is known about the early response genes. We have extended these earlier findings to characterize more fully the early transcriptional response at 15, 30, and 60 minutes of cold shock at 13°C and also the response to recovery after cold shock for 30 and 60 minutes at 30°C using DNA microarrays. After 60 minutes of recovery from cold shock, gene expression returned to pre‐cold shock levels. Results were analyzed using the program GenMAPP to determine which biological pathways and processes were activated in response to cold shock and recovery. We found that genes involved in ribosome biogenesis were induced by cold shock and then repressed during the recovery phase. These results were confirmed through quantitative reverse transcriptase polymerase chain reaction (qRT‐PCR) experiments to detect the expression of known ribosome biogenesis genes such as NSR1. This work was supported by a Loyola Marymount University Kadner‐Pitts Research Grant (K.D.D.) and NSF award 0921038 (B.A.I., K.M.B., and K.D.D.).