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Sem1 is required for TREX2 stability
Author(s) -
Oliver Encarna García,
García Pau Pascual,
Navarro Susana Rodríguez
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.831.2
Subject(s) - proteasome , component (thermodynamics) , biology , mutant , messenger rna , microbiology and biotechnology , gene , genetics , physics , thermodynamics
Sem1 is a conserved component of the lid subcomplex of the 19S regulatory particle of the 26S proteasome. Several studies have established links between components of the mRNA export machinery and Sem1 in a proteasome‐independent manner. Here we provide additional data that support this functional and physical association. We show that Sem1 is genetically linked to Sus1, a bona fide component of TREX2 complex. Biochemical analyses revealed that, Sem1 and Sus1 interact physically, which depends on the presence of Sac3. Reciprocally Sus1‐Sac3 interaction depends on Sem1. Moreover, mRNA export is drastically inhibit in the double mutant sus1Δsem1Δ . Here we will present new data concerning Sem1 proteasome‐independent role in mRNA export as a functional component of the TREX2 complex.