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Efficient myogenic commitment of hESCs and iPSC‐derived cells on biomimetic materials replicating myoblast topography
Author(s) -
Hwang Nathaniel Sukyeon,
Langer Robert S
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.824.5
Subject(s) - induced pluripotent stem cell , microbiology and biotechnology , embryonic stem cell , mesenchymal stem cell , myocyte , skeletal muscle , cell , biology , cellular differentiation , anatomy , genetics , gene
Recent development in stem cell technology has demonstrated human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) as possible cells for cell‐based therapy and studies of particular differentiation program. However, spontaneous and heterogeneous differentiation of these cells may limit the potential use. Here, we describe the efficient generation of mesenchymal cell population from both hESCs and iPSCs having non‐turmoigenic potentianl and their commitment into myoblast. In skeletal development, increased cell‐cell contact plays critical initial steps for myogenic commitment. We demonstrate that iPSCs and hESCs‐derived cells can undergo efficient myogenic commitment by topographical cues present in their environment. We have created substrates from biomimetic materials that can replicate the mico‐ and nanoscale topography of fully differentiated skeletal myoblast. When hESCs and iPSCs‐derived mesenchymal cells were cultured on biomimetic pattern, mesenchymal cells followed the underying myoblast pattern. Furthermore, gene expression and cell fusion index showed enhanced myogenic commitment on these substrates. These results demonstrates that myogenic potential of hESCs and iPSCs‐derived cells are highly dependent on the micro‐ and nanoscale topographical cues provide by skeletal myoblast. Grant Funding Source : NIDCR (NIH)