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Oxygen concentration modulates in vitro differentiation of porcine muscle derived stem cells
Author(s) -
Loughna Paul Thomas,
Redshaw Zoe
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.824.2
Subject(s) - myogenesis , adipogenesis , microbiology and biotechnology , stem cell , progenitor cell , in vitro , cellular differentiation , myod , chemistry , myocyte , myosin , biology , skeletal muscle , anatomy , biochemistry , mesenchymal stem cell , gene
The regenerative properties of skeletal muscle are attributed to the resident myogenic progenitor cells known as satellite cells. They are reportedly heterogeneous in nature, with regard to their proliferation, fusion and transplantation abilities. Most in vitro studies to date have examined the differentiation of rodent satellite cells at atmospheric oxygen (O2) concentration. Our work focuses on the isolation of satellite cells from the pig, comparing differentiation at atmospheric (21%) with physiological (5%) O2 levels. Cells derived from two distinct skeletal muscles, the diaphragm (D) and hind limb semi‐membranosus (SM) muscles differ in their capacity to undergo myogenesis and adipogenesis in vitro. D cells exhibited greater myogenic ability compared with those from SM, while the converse was true for adipogenesis. The myogenic ability of cells isolated from D as measured by expression of desmin, myosin heavy chain and myotube number was significantly enhanced at 5% compared to 21% O2. The myotubes formed were significantly larger, with greater nuclei number at the lower O2 concentration. In contrast, when encouraged towards an adipogenic fate, formation of adipocytes in SM cells was reduced at the lower O2 level. These data demonstrate that stem cells derived from metabolically distinct muscles exhibit different lineage potentials, which can be influenced by O2 concentration.