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Upregulation of Sca‐1 in satellite cells during diaphragmatic skeletal muscle regeneration: Potential novel mechanism of skeletal muscle regeneration
Author(s) -
Rezk Bashir Mahmoud,
Yoshida Tadashi,
SemprunPrieto Laura,
Sukhanov Sergiy,
Delafontane Patrice
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.824.1
Subject(s) - skeletal muscle , myogenesis , regeneration (biology) , downregulation and upregulation , myosin , biology , endocrinology , myocyte , medicine , microbiology and biotechnology , immunostaining , chemistry , immunology , immunohistochemistry , biochemistry , gene
Stem cell antigen‐1 (Sca‐1) is an 18kDa glycosyl phosphatidylinositol‐anchored cell surface protein of the Ly‐6 family that is involved in cell activation, proliferation and differentiation. Regeneration of skeletal muscle is a multifactorial process which includes proliferation and differentiation of satellite cells into new muscle fibers. Mechanisms of skeletal muscle regeneration, however, are poorly understood. We previously reported that angiotensin II (Ang II) is implicated in the induction of skeletal muscle atrophy that is characteristic of advanced heart failure. This study was designed to investigate the effects of Ang II on diaphragmatic skeletal muscle and determine mechanism(s) of skeletal muscle regeneration. FVB mice were infused with 1μg/Kg/min Ang II or sham‐infused for one week. Immunostaining for embryonic myosin heavy chain revealed multiple areas of regenerating muscle fibers and Sca‐1 expression was markedly upregulated in the satellite cells of these injured areas. Both proliferation markers PCNA and Ki67 were upregulated as detected by Western blot for PCNA and immunostaining for Ki67. The myogenic factor, M‐cadherin was also increased in the injured muscle. In conclusion, our results demonstrate that Ang II induced injury of diaphragmatic skeletal muscle is accompanied by increased proliferation and myogenesis that is potentially mediated, at least in part, by increased activity of Sca‐1 in muscle satellite cells. Grant Funding Source: NIH