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Rotational Stress Impairs Cutaneous Wound Healing In Mice
Author(s) -
MonteAltoCosta Andréa,
RomanaSouza Bruna
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.823.4
Subject(s) - myofibroblast , wound healing , granulation tissue , lesion , pathology , medicine , andrology , anatomy , surgery , fibrosis
Objective The aim of this study was to investigate the effects of the rotational stress on cutaneous wound healing in mice. Methods Male mice were spun at 45rpm for 15minutes every hour from three days before wounding until euthanasia. Control animals were not submitted to stress. A full‐thickness lesion was created and measured. Fourteen days after, animals were killed and lesions were collected. Sections were stained with HE and immunostained against F4/80 and alpha‐smooth muscle actin to quantify macrophages and myofibroblasts, respectively. Data are expressed as mean±S.E.M and analyzed by Student's t test. Results Fourteen days after wounding, wound area was greater in the stress group than in the control group (p<0.05), while re‐epithelialized wound area was smaller in the stress group than in the control group (p<0.05). Stressed mice presented less organized collagenous granulation tissue (rich in inflammatory cells) when compared with control mice. The macrophages number and myofibroblasts density was greater in the stress group than in the control group (p<0.05). Conclusion The rotational stress delays the cutaneous wound healing in mice.