Premium
The characteristics of rabbit and rat mesenchymal stromal cell growth and attachment to mesh used in hernia repair
Author(s) -
Fagan Diana L.,
Lydic Melissa,
Marie Hazel,
Fredette Paul,
Heffner Jeremy,
Dorion Heath
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.823.3
Subject(s) - mesenchymal stem cell , cell growth , wound healing , stromal cell , titer , andrology , rabbit (cipher) , cell , chemistry , biology , microbiology and biotechnology , immunology , pathology , medicine , biochemistry , computer science , computer security , antibody
The goal of this project is to create an optimal environment for wound healing. Preliminary results from a rabbit model suggest that the addition of mesenchymal stromal cells (MSC) to a repaired incision improves the biomechanical properties of the wound. To increase sample size and reproducibility, these studies will be repeated in an inbred rat model. A review of the literature shows a wide variety of culture conditions used for rat MSCs. This study compares proliferation of rabbit and rat MSCs when grown in ten different growth medias. The Cell Titer 96 ® Cell Proliferation Assay and direct cell counts were used to measure the proliferation of MSC's. Rabbit and rat MSC growth was optimal in alpha MEM + 20% FCS or Hams F12 + 10% FCS. To prepare for the extension of these studies into the rat model, four mesh materials commonly used in hernia repair were tested to determine the best vehicle for the addition of MSCs. MSCs were incubated with the mesh for 30 min at 37 o C. Non‐adherent cells were removed and the remaining cell number determined by incubation with Cell Titer 96 ® solution and comparison to an MSC standard curve. Optimal adherence was seen with Bard ® CollaMend (38% of added cells). Future studies will use Bard ® CollaMend as a vehicle when adding MSCs to a repaired abdominal incision. Supported by St. Elizabeth Health Center and Youngstown State University.