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Albumingel for cartilage and intervertebral disc regeneration prevents blood vessel formation
Author(s) -
Scholz Beate,
Kinzelmann Claudia,
Benz Karin,
Baumer Yvonne,
Mollenhauer Jürgen,
Schlosshauer Burkhard
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.823.2
Subject(s) - cartilage , implant , regeneration (biology) , blood vessel , chondrocyte , matrix (chemical analysis) , hyaluronic acid , microbiology and biotechnology , chemistry , anatomy , pathology , medicine , andrology , surgery , biology , chromatography
Healthy cartilage is devoid of blood vessels, thus an implant matrix for autologous chondrocyte implantation should exhibit antiangiogenic properties. The purpose of the work was to investigate the influence of an albumin gel (AG) for cartilage and intervertebral disc (IVD) regeneration on blood vessel formation. The AG is composed of activated sheep serum albumin, hyaluronic acid and a polyethylene glycol spacer. Primary human endothelial cells (EC) cultured on the AG did not adhere on the surface and did not proliferate. Life‐dead staining displayed an increase in dead cells within 5 days of culture on the AG, while extracts of the AG showed no cytotoxic effect. Controversely, the vast majority of human primary chondrocytes cultured within the AG were vital after 5 days. EC stimulated by vascular endothelial growth factor did hardly invade through AG coated transwell filter. Blood vessels of the chick chorioallantoic membrane did not grow into the implanted AG. Additionally, IVD cells enclosed in the AG were implanted subcutaneously in immunodeficient mice. After 2 weeks there was no evidence of blood vessel formation in the implant. The results indicate that the AG is a suitable 3D‐matrix for chondrocytes exhibiting nonpermissive properties for EC and therefore could be an ideal implant for cartilage and IVD regeneration. All procedures were approved by the local Ethics and the local Animal Care and Use Committee.