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Aging causes accumulation of non‐proliferative adipose derived stem cells that is attenuated by caloric restriction in mice
Author(s) -
Schmuck Eric,
Mulligan Jacob D,
Saupe Kurt W
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.823.13
Subject(s) - stem cell , adipose tissue , cd34 , mesenchymal stem cell , white adipose tissue , biology , progenitor cell , cdkn2a , cancer research , endocrinology , medicine , andrology , microbiology and biotechnology , cancer
While white adipose tissue (WAT) is a promising source of mesenchymal stem cells, the effects of WAT remodeling factors such as aging and diet on these cells are unknown. To address this adipose‐derived stem cells (ADSC), defined as the non‐adipocyte cell fraction that is Lin‐/CD34+/CD31‐, were isolated from epididymal fat pads of adult (10–12 months, n=12) and aged (>24months, n=12) C57BL/6 mice fed ad lib or a calorie restricted (CR) diet known to retard aging and tumor development. ADSC abundance, single cell clonality, expression of stem cell associated genes and enzymatic activities were quantified. Aging increased ADSC abundance 3.2 fold and expression of the genes Cdkn2a 9.8 fold and Isl1 60.6 fold. However, clonality was decreased 33% suggesting that aging causes accumulation of non‐replicative ADSC. CR attenuated the age‐associated increase in ADCS abundance by greater than 50%, but decreased clonality to 10% and 7% of levels in adult and aged ad lib groups respectively. Therefore, CR appears to have anti‐proliferative effects on ADSC that may be advantageous from the perspective of cancer, but disadvantageous for regenerative medicine applications. Grant Funding Source : 1R21HL092477‐01 Proposal Title: Impact of chronic caloric restriction on resident progenitor cells in the heart