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Phytoestrogenic effects of catalpol in T47D and MDA‐MB231 cells in culture
Author(s) -
Hao Qingxiu,
Niu Jianzhao,
Wang Jifeng,
Zhao Piwan
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.821.9
Subject(s) - catalpol , estrogen receptor , estrogen , messenger rna , medicine , cell growth , endocrinology , chemistry , biology , microbiology and biotechnology , cancer , gene , breast cancer , biochemistry , stereochemistry , glycoside
The phytoestrogenic effects of catalpol were measured by the proliferation and cell cycle, expressions of ERá/ERâ protein and ps2 mRNA on breast cancer cells T47D cells MDA‐MB‐231 cells. Result: (1) The cells proliferation was enhanced significantly by treatment with catalpol in the range of 10‐7 to 10‐6 M for breast T47D cells. The proliferation effects were inhibited by adding anti‐estrogen ICI182 780 (10‐8M). (2) There was no significant difference on the proliferation of MDA‐MB‐231 cells by treatment with tested concentration of catalpol as well as by co‐treatment with anti‐estrogen ICI182 780(10‐8M) compared with solvent control group. (3) Catalpol up‐regulated pS2 mRNA expression of T47D cells at 10‐7M or 10‐6M and they increased the level in T47D cells, ERá protein expression as well. 10‐6M catalpol increased the level of ERâ protein expression in T47D cells. However, 10‐7 M catalpol did not show remarkable effects to the level of ERâ protein expression in T47D cells. Conclusion: Catalpol possessed phytoestrogenic effects by up‐regulating pS2 gene expression and the receptor subtype of ERá and ERâ expression. The phytoestrogenic effects were mediated by regulating ER. Supported by the 111 project B70007, P.R. China and the International Science and Technology Cooperation Program of China, 2008DFA31970 to Niu J.Z.