Premium
Soybean phytoestrogens modulate ion transport in porcine endometrial epithelial cells
Author(s) -
Deachapunya Chatsri,
Poonyachoti Sutthasinee
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.821.4
Subject(s) - genistein , daidzein , bumetanide , phytoestrogens , chemistry , dids , amiloride , ussing chamber , medicine , endocrinology , apical membrane , trpc , forskolin , ion transporter , biochemistry , secretion , biology , transient receptor potential channel , receptor , estrogen , organic chemistry , membrane , sodium
Effects of the phytoestrogens genistein and daidzein on ion transport functions in primary cultured porcine endometrial epithelial cells were investigated using the Ussing chamber technique. Acute exposure of 17β‐estradiol failed to change short circuit current (Isc) whereas genistein produced a concentration‐dependent increase in Isc (EC 50 37 μM). The genistein‐stimulated Isc was diminished in Cl − ‐free solution or in the presence of bumetanide, but not amiloride. In amphotericin B‐permeabilized monolayers, genistein activated apical NPPB‐ and glibenclamide‐senstive Cl − current with no effect on apical K + current. Genistein also activated basolateral barium‐sensitive K + current which created a driving force for Cl − secretion. Treatment of monolayers with Ca 2+ chelating BAPTA‐AM did not affect the genistein‐activated Isc, suggesting a Ca 2+ ‐independent regulation of Cl − and K + channels. In contrast, daidzein decreased Isc by inhibiting apical Cl − current with no change in basolateral K + current. These data suggest that the Cl − secretory pathway in endometrial epithelial cells is subject to be regulated by soybean phytoestrogens genistein and daidzein. This work was supported by The Thailand Research Fund (RMU 4980026).