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Nanoparticle Internalization and Transport by Caveolae
Author(s) -
Wang Zhenjia,
Tiruppathi Chinnaswamy,
Minshall Richard D,
Malik Asrar B
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.820.1
Subject(s) - caveolae , internalization , nanoparticle , bovine serum albumin , biophysics , transcytosis , chemistry , microbiology and biotechnology , endothelial stem cell , albumin , nanotechnology , materials science , membrane , endocytosis , biochemistry , cell , biology , in vitro
Caveolae are flask‐shaped membrane invaginations in endothelial cells involved in uptake of macromolecules such as albumin and viral particles. It is unknown whether caveolae can internalize nanoparticles. Here we investigated uptake of nanoparticles of different sizes ranging from 20 nm to 100 nm in endothelial cell monolayers. We observed that BSA (bovine serum albumin) coating of nanoparticles was required for the internalization of nanoparticles and the uptake is dependent on particle size. Co‐localization of caveolae staining and BSA‐coated nanoparticles indicated nanoparticles were internalized in endothelial cells via caveolae. Using a nanoruler made of a dual‐color nanoparticle pair to measure caveolae size and assembly dynamics in live endothelial cells, we found that only nanoparticles less than caveolae size were internalized. Uptake of nanoparticle cargo in caveolae was followed by its transport across the endothelial monolayer via caveolae. Transport was dependent on size of nanoparticles; e.g., transport of 40 nm particles was 2‐fold greater than 100 nm particles. The present results suggest that nanoparticles of a particular size (~40nm diam) coated with albumin provide an efficient means of delivering therapeutic proteins across the vascular endothelial barrier.