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Mre11 expression in mitochondria of renal cells
Author(s) -
Dmitrieva Natalia I.,
Malide Daniela,
Burg Maurice B.
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.817.4
Subject(s) - cytoplasm , mitochondrion , microbiology and biotechnology , nucleus , biology , chemistry
Mre11 is a key component of the nuclear DNA damage repair network. Nevertheless, thermal stress causes translocation of some of the nuclear Mre11 into the cytoplasm, and we previously found that high NaCl does also. The location of the Mre11 in cytoplasm has been unknown. Noting that Mre11 was identified in yeast mitochondria, we searched for Mre11 in mitochondria of mammalian cells under various conditions. Mre11 is present in the mitochondria isolated from mIMCD3 cells (Western blot), and the amount in the mitochondria increases when NaCl bathing the cells is elevated. In non synchronized primary kidney cells Mre11 forms foci that colocalize with mitochondria (Confocal microscopy). When the primary kidney cells are grown in Matrigel (BD Biosciences), they differentiate and form tubules. In non differentiated cells Mre11 is mostly in the nucleus, but it is mostly in the cytoplasm in cells that have formed tubules. In sections from mouse kidney we find abundant MRE11 colocalized with mitochondria in the cytoplasm of mitochondrial rich proximal tubule and thick ascending limb cells. In collecting ducts, which contain fewer mitochondria, MRE11 is concentrated in nuclei. We conclude that 1) Mre11 localizes in mitochondria as well as in the nuclei of renal cells and 2) its distribution varies depending on the stress to which the cells are exposed and on their differentiation. Supported by the Intramural Program of NHLBI.